Terminal Deoxynucleotidyl Transferase, Recombinant from Promega

Product Specs
ItemTerminal Deoxynucleotidyl Transferase, Recombinant
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM1871
FormatConcentration: 30u/µl.
The enzyme is a single protein of approximately 43kDa with =90% purity.
Conjugate/Tag/LabelUnconjugated
ActivityOne unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of dATP to p(dA)50 in 60 minutes at 37°C.
Molecule NameTerminal Deoxynucleotidyl Transferase
Source/Expression SystemRecombinant
Quantity300u
Molecular Weight~83kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2CellsDecember 22, 2022Key J, Gispert S, Koornneef L, Sleddens-Linkels E, Kohli A, Torres-Odio S, Koepf G, Amr S, Reichlmeir M, Harter PN, et al.5 min wash in distilled water. Thereafter, the slides were washed for 5 min in TdT-buffer (Promega, M1871) and incubated for 2 h in TdT-buffer containing 0.01 mM Biotin-16-dUTP (Roche, Basel, Switzerland, 11093070910)

(2) Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse ModelMethods in Molecular BiologyNovember 16, 2022Taylor A. Stepien, Stephen J. Libby, Joyce E. Karlinsey, Michael A. Brehm, Dale L. Greiner, Leonard D. Shultz, Thea Brabb, Ferric C. Fang0.5 mM ddCTP prepared as follows: Terminal Deoxynucleotidyl Transferase, Recombinant (Promega Cat#M1871). Performa DTR Gel Filtration Cartridges (EdgeBio Cat#98780). Qubit dsDNA BR Assay Kit (Invitrogen

(3) Chd1 protects genome integrity at promoters to sustain hypertranscription in embryonic stem cellsNature communicationsAugust 11, 2021Bulut-Karslioglu A, Jin H, Kim YK, Cho B, Guzman-Ayala M, Williamson AJK, Hejna M, Stötzel M, Whetton AD, Song JS, et al.labeling and control reactions. Nuclei were resuspended in 625 μl 1x TdT buffer with 2.5 μl TdT (Promega, M1871) and 2 μl biotin-16-dUTP (Roche 11093070910), and were incubated at 37 °C for 1 h. Control reaction

(4) The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicleeLifeApril 27, 2020Kantarci H, Gou Y, Riley BB.IgG (ThermoFisher Scientific A-11003/A-11010, 1:50). Promega terminal deoxynucleotidyl transferase (M1871) was used according to manufacturer’s protocol to perform the TUNEL assay. Cell transplantation Wild-type

(5) Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytesMitosis and Meiosis Part BJune 30, 2018Pierre Romé, Hiroyuki Ohkuramolecules (such as Alexa 546-dUTP) and 1.5 unit/μl of terminal deoxynucleotidyl transferase (TdT; #M1871 Promega) in transferase buffer. The solution is incubated at 37°C for one hour before inactivating TdT

(6) Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesisAutophagyJanuary 1, 2018Yamaguchi J, Suzuki C, Nanao T, Kakuta S, Ozawa K, Tanida I, Saitoh T, Sunabori T, Komatsu M, Tanaka K, et al.Tesque, 29442–85) were incubated with 150 U/ml terminal deoxynucleotidyl transferase (TdT; Promega, M1871) and 10 nmol/ml Biotin-16-UTP (Roche, 11093070910) in TdT buffer (100 mM sodium cacodylate, pH 7.0,

(7) Modeling Enzyme Processivity Reveals that RNA-Seq Libraries Are Biased in Characteristic and Correctable WaysCell SystemsNovember 23, 2016Nathan Archer, Mark D. Walsh, Vahid Shahrezaei, Daniel HebenstreitDeoxynucleotide (dNTP) Solution Set,New England Biolabs,Part#N0446S Terminal deoxynucleotidyl transferase (TdT),Promega,Cat#M1871 NP-40,ThermoFisher,Cat#28324 Agencourt Ampure XP Beads,Beckman Coulter,Part#A63881 polyA Spin mRNA Isolation Kit

(8) Spemann organizer gene Goosecoid promotes delamination of neuroblasts from the otic vesicleProceedings of the National Academy of Sciences of the United States of AmericaNovember 1, 2016Kantarci H, Gerberding A, Riley BB.A-11003/ A-11010, 1:50). TUNEL assay was performed by using Promega terminal deoxynucleotidyl transferase (M1871) according to the manufacturer’s protocol. Whole-mount stained embryos were prepared for cryosectioning as

(9) Avian Models in Teratology and Developmental ToxicologyMethods in Molecular BiologyAugust 30, 2015Susan M. Smith, George R. Flentke, Ana Garicbuffer + 10 μl 10 mM CoCl2 + 4 μl 2 mM BrdUTP (Sigma B-0631) + 2 μl TdT enzyme (5 units/μl, Promega #M1871). Use 200 μl per slide, made fresh immediately before use. The final composition is 1x TdT buffer, 1

(10) Activating immunity in the liver. I. Liver dendritic cells (but not hepatocytes) are potent activators of IFN-gamma release by liver NKT cellsThe Journal of ImmunologyAugust 1, 2001Zlatko Trobonjaca, Frank Leithäuser, Peter Möller, Reinhold Schirmbeck, Jörg ReimannLouis, MO) for 15 min at 37°C. The labeling reaction was conducted using 10 U of TdT (catalogue no. M1871; Promega, Madison, WI) and 17 ␮M biotin-16-dUTP (catalogue no. 1093070; Boehringer Mannheim, Mannheim,

(11) In Situ Characterization of Genetically Targeted (Green Fluorescent) Single Cells and Their Microenvironment in an Adoptive HostThe American Journal of PathologyJune 8, 2001Frank Leithäuser, Zlatko Trobonjaca, Jörg Reimann, Peter MöllerSigmaAldrich) for 15 minutes at 37°C. The labeling reaction was performed using 10 U of TdT (catalog no. M1871; Promega, Mannheim, Germany) and 20 ␮mol/L of biotin-16dUTP (catalog no. 1093070, Boehringer-Mannheim)

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